藏药现代化团组“利用高速逆流色谱和大孔树脂快速富集和分离唐古特大黄中的三种蒽醌苷和一种二苯乙烯苷类化合物”一文在Journal of Chromatography B上发表

　　陈涛,刘永玲,陈晨,邹登朗,尤进茂,孙菁*,李玉林*.Application of high-speed counter-current chromatography combined with macroporous resin for rapid enrichment and separation of three anthraquinone glycosides and one stilbene glycoside from Rheum tanguticum. Journal of Chromatography B, 2014,957, 90-95.

　　该研究首先利用大孔树脂快速富集到三种蒽醌苷和一种二苯乙烯苷类化合物，然后采用高速逆流色谱高效分离得到了四种目标化合物。方法简便、高效，非常适合于四种对照品化合物的分离制备。

　　Abstruct: In this paper, an efficient method was successfully established by the combination of macroporous resin (MR) and high-speed counter-current chromatography (HSCCC) for rapid enrichment and separation of aloe-emodin8-O-β-D-glucoside, emodin 1-O-β-D-glucoside, emodin 8-O-β-D-glucoside and piceatannol 4-O-β-D-(6’’-O-gallate)-glucoside. Six kinds of macroporous resins were investigated in the first step and X-5 macroporous resin was selected for the enrichment of the target compounds. The recoveries of the target compounds reached 89.0, 85.9, 82.3 and 84.9% respectively after 40% ethanol elution. In the second step, the target compounds were separated by HSCCC with a two-phase solvent system composed of chloroform/ethyl acetate/methanol/water (8:1:6:5, v/v). The established method will be helpful for further characterization and utilization of Rheum tanguticum. The results demonstrate that MR coupled with HSCCC is a powerful technique for separation of bioactive compounds from natural products.