SEMI-PREPARATIVE SEPARATION AND PURIFICATION OF THREE FLAVONOIDS FROM PEDICULARIS LONGIFLORA VAR.TUBIFORMIS (KLOTZSCH) P. C. TSOONG BY HSCCC

　　赵晓辉,韩发,李玉林,周国英,岳会兰*.SEMI-PREPARATIVE SEPARATION AND PURIFICATION OF THREE FLAVONOIDS FROM PEDICULARIS LONGIFLORA VAR.TUBIFORMIS (KLOTZSCH) P. C. TSOONG BY HSCCC.Journal of Liquid Chromatography & Related Technologies.2013,36:1751–1761.IF=0.565

　　Abstract:An effective high–speed counter–current chromatography (HSCCC) method was established for semi-preparative isolation and purification of bioactive chemical constituents from the Tibetan medicinal plant Pedicularis longiflora var. tubiformis (Klotzsch) P. C. Tsoong. With a two-phase solvent system composed of chloroform–methanol–water (8:4:5, v=v), 40 mg of extract was separated to yield luteolin (12.5 mg), apigenin (9.6 mg), and chrysoeriol (4.8 mg), with purities of 99.3, 98.2, and 98.6%, respectively, as determined by high-performance liquid chromatography (HPLC). The chemical structures of these three components were identified by 1H NMR and 13C NMR.