Separation of Phenylpropanoid Glycosides from a Chinese Herb by HSCCC

　　陈晨,赵晓辉*,岳会兰,李玉林,陈涛.Separation of Phenylpropanoid Glycosides from a Chinese Herb by HSCCC.Journal of Chromatographic Science.2013:1–5.IF=0.794

　　Abstract:An effective high-speed counter-current chromatography method was established for the preparative isolation and purification of two phenylpropanoid glycosides from the Tibetan medicinal plant Pedicularis longiflora Rudolph. var. tubiformis (Klotz). Tsoong. With a two-phase solvent system composed of chloroform–n-butanol–methanol–water(4:3:4:5, v/v), 40 mg of an extract of Pedicularis longiflora Rudolph.var. tubiformis (Klotz). Tsoong was separated to yield 20 mg of verbascosideand 18 mg of isoacteoside, with purity values of 97 and 98%,respectively. The chemical structures of these two components were identified by proton and carbon nuclear magnetic resonance. In addition,the antioxidant activity of the two phenylpropanoid glycosides was evaluated by 2,2-diphenyl-1-picrylhydrazyl (DPPH)–high-performance liquid chromatography, and the results showed that the two components exhibited strong antioxidant DPPH radical scavenging activity, with IC50 values of 15.6 and 18.9 mg/mL, respectively.